Recent Advances in Ciliate Biology. Howard-Till RA, Kar UP, Fabritius AS, Winey M. Annu Rev Cell Dev Biol. 2022. PMID: 35901313


Electron cryo-tomography structure of axonemal doublet microtubule from Tetrahymena thermophila. Li S, Fernandez JJ, Fabritius AS, Agard DA, Winey M.Life Sci Alliance. 2021 Dec 30;5(3):e202101225. doi: 10.26508/lsa.202101225. Print 2022 Mar.PMID: 34969817

Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null Tetrahymena cells reveals functional MIPs. Fabritius AS, Bayless BA, Li S, Stoddard D, Heydeck W, Ebmeier CC, Anderson L, Gunnels T, Nachiappan C, Whittall JB, Old W, Agard DA, Nicastro D, Winey M. Mol Biol Cell. 2021 Nov 1;32(21):br8. doi: 10.1091/mbc.E20-12-0786. Epub 2021 Aug 18.PMID: 34406789

Tetrahymena Poc5 is a transient basal body component that is important for basal body maturation. Heydeck W, Bayless B, Stemm-Wolf A, O’Toole E, Fabritius A, Ozello C, Nguyen M, Winey M. J Cell Science. 2020 Jun 4;133(11).

Yeast pericentrin/Spc110 contains multiple domains required for tethering the gamma-tubulin complex to the centrosome. Alonso A, Fabritius A, Ozello C, Andreas M, Klenchin D, Rayment I, Winey M. Mol Biol Cell. 2020 July1;31(14):1437-1452.

Microtubule glycylation promotes attachment of basal bodies to the cell cortex. Junker A, Son A, O’Toole E, Meehl J, Guha M, Winey M, Honts J, Gaertig J, Pearson C. J Cell Science. 2019 Aug7;132(15)

Motile Cilia: Innovation and Insight from ciliate model organisms. Bayless, A, Navarro F, Winey M. Frontiers in Cell and Developmental Biology. 2019; 7: 265.

Tetrahymena RIB72A and RIB72B are Microtubule Inner Proteins in the ciliary doublet microtubules. Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D, Suryawanshi S, Tomasi RF, Dupuis-Williams P, Baroud  CN, Gaertig J, Winey M, Nicastro D. Mol Biology Cell. 2018 Aug22.

Key phosphorylation events in Spc29 and Spc42 guide multiple steps of yeast centrosome duplication.Jones MH, O’Toole ET, Fabritius AS, Muller EG, Meehl JB, Jaspersen SL, Winey M. Mol Biol Cell. 2018. Sep 15;29(19):2280-2291

The molecular architecture of the yeast spindle pole body core determined by Bayesian integrative modeling. Viswanath S, Bonomi M, Kim SJ, Klenchin VA, Taylor KC, Yabut KC, Umbreit NT, Van Epps HA, Meehl J, Jones MH, Russel D, Velazquez-Muriel JA, Winey M, Rayment I, Davis TN, Sali A, Muller EG. Mol Biol Cell. 2017 Nov 7;28(23):3298-3314.

Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast. O’Toole ET, Giddings TH Jr, Winey M. Cold Spring Harb Protoc. 2017 Mar 1;2017(3)

Cryopreparation and Electron Tomography of Yeast Cells. O’Toole ET, Giddings TH Jr, Winey M. Cold Spring Harb Protoc. 2017 Mar 1;2017(3)

Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies. Heydeck W, Stemm-Wolf AJ, Knop J, Poh CC, Winey M.  mSphere. 2016 Nov 16;1(6).

Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity. Meehl JB, Bayless BA, Giddings TH Jr, Pearson CG, Winey M. Mol Biol Cell. 2016 Aug 1;27(15):2394-403.

Identifying domains of EFHC1 involved in ciliary localization, ciliogenesis, and the regulation of Wnt signaling. Zhao Y, Shi J, Winey M, Klymkowsky MW. Dev Biol. 2016 Mar 15;411(2):257-265.

Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication. Burns S, Avena JS, Unruh JR, Yu Z, Smith SE, Slaughter BD, Winey M, Jaspersen SL. Elife. 2015 Sep 15.

Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus. Shi J, Zhao Y, Vonderfecht T, Winey M, Klymkowsky MW. Sci Rep. 2015 May 27;5:10283.

Metallothionein as a clonable tag for protein localization by electron microscopy of cells. Morphew MK, O’Toole ET, Page CL, Pagratis M, Meehl J, Giddings T, Gardner JM, Ackerson C, Jaspersen SL, Winey M, Hoenger A, McIntosh JR. J Microsc. 2015 Oct;260(1):20-9.

Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning. Peng Y, Moritz M, Han X, Giddings TH, Lyon A, Kollman J, Winey M, Yates J 3rd, Agard DA, Drubin DG, Barnes G. Mol Biol Cell. 2015 Jul 1;26(13):2505-18.

The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes. Waters AM, Asfahani R, Carroll P, Bicknell L, Lescai F, Bright A, Chanudet E, Brooks A, Christou-Savina S, Osman G, Walsh P, Bacchelli C, Chapgier A, Vernay B, Bader DM, Deshpande C, O’ Sullivan M, Ocaka L, Stanescu H, Stewart HS, Hildebrandt F, Otto E, Johnson CA, Szymanska K, Katsanis N, Davis E, Kleta R, Hubank M, Doxsey S, Jackson A, Stupka E, Winey M, Beales PL. J Med Genet. 2015 Mar;52(3):147-56.

Licensing of yeast centrosome duplication requires phosphoregulation of sfi1. Avena JS, Burns S, Yu Z, Ebmeier CC, Old WM, Jaspersen SL, Winey M. PLoS Genet. 2014 Oct 23;10(10):e1004666. doi: 10.1371/journal.pgen.1004666. eCollection 2014 Oct.

Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle. Nannas NJ, O’Toole ET, Winey M, Murray AW. Mol Biol Cell. 2014 Dec 15;25(25):4034-48.

Minus-end-directed Kinesin-14 motors align antiparallel microtubules to control metaphase spindle length. Hepperla AJ, Willey PT, Coombes CE, Schuster BM, Gerami-Nejad M, McClellan M, Mukherjee S, Fox J, Winey M, Odde DJ, O’Toole E, Gardner MK. Dev Cell. 2014 Oct 13;31(1):61-72.

Chibby functions in Xenopus ciliary assembly, embryonic development, and the regulation of gene expression. Shi J, Zhao Y, Galati D, Winey M, Klymkowsky MW. Dev Biol. 2014 Nov 15;395(2):287-98. doi: 10.1016/j.ydbio.2014.09.008. Epub 2014 Sep 16.

Function of the male-gamete-specific fusion protein HAP2 in a seven-sexed ciliate. Cole ES, Cassidy-Hanley D, Fricke Pinello J, Zeng H, Hsueh M, Kolbin D, Ozzello C, Giddings T Jr, Winey M, Clark TG. Curr Biol. 2014 Sep 22;24(18):2168-2173.

Membrane dynamics at the nuclear exchange junction during early mating (one to four hours) in the ciliate Tetrahymena thermophila. Cole ES, Giddings TH Jr, Ozzello C, Winey M, O’Toole E, Orias J, Hamilton E, Guerrier S, Ballard A, Aronstein T. Eukaryot Cell. 2015 Feb;14(2):116-27.

Centriole structure. Winey M, O’Toole E. Philos Trans R Soc Lond B Biol Sci. 2014 Sep 5;369(1650).

Conventional transmission electron microscopy. Winey M, Meehl JB, O’Toole ET, Giddings TH Jr. Mol Biol Cell. 2014 Feb;25(3):319-23.

Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly. Nazarova E, O’Toole E, Kaitna S, Francois P, Winey M, Vogel J. Mol Biol Cell. 2013 Oct;24(20):3238-50.

Genome-wide haploinsufficiency screen reveals a novel role for γ-TuSC in spindle organization and genome stability. Choy JS, O’Toole E, Schuster BM, Crisp MJ, Karpova TS, McNally JG, Winey M, Gardner MK, Basrai MA. Mol Biol Cell. 2013 Sep;24(17):2753-63.

ε-tubulin is essential in Tetrahymena thermophila for the assembly and stability of basal bodies. Ross I, Clarissa C, Giddings TH Jr, Winey M. J Cell Sci. 2013 Aug 1;126(Pt 15):3441-51. doi: 10.1242/jcs.128694. Epub 2013 May 23.

Activation of the yeast Hippo pathway by phosphorylation-dependent assembly of signaling complexes. Rock JM, Lim D, Stach L, Ogrodowicz RW, Keck JM, Jones MH, Wong CC, Yates JR 3rd, Winey M, Smerdon SJ, Yaffe MB, Amon A. Science. 2013 May 17;340(6134):871-5.

Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in Tetrahymena thermophila. Stemm-Wolf AJ, Meehl JB, Winey M. J Cell Sci. 2013 Apr 1;126(Pt 7):1659-71.

Mps1 and Ipl1/Aurora B act sequentially to correctly orient chromosomes on the meiotic spindle of budding yeast. Meyer RE, Kim S, Obeso D, Straight PD, Winey M, Dawson DS. Science. 2013 Mar 1;339(6123):1071-4.

Bld10/Cep135 stabilizes basal bodies to resist cilia-generated forces. Bayless BA, Giddings TH Jr, Winey M, Pearson CG. Mol Biol Cell. 2012 Dec;23(24):4820-32.

The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies. Vonderfecht T, Cookson MW, Giddings TH Jr, Clarissa C, Winey M. Mol Biol Cell. 2012 Dec;23(24):4766-77.

Bioleaching of fly ash by the tropical marine yeast, Yarrowia lipolytica NCIM 3589. Bankar A, Winey M, Prakash D, Kumar AR, Gosavi S, Kapadnis B, Zinjarde S. Appl Biochem Biotechnol. 2012 Dec;168(8):2205-17.

Mitotic spindle form and function. Winey M, Bloom K. Genetics. 2012 Apr;190(4):1197-224.

The MPS1 family of protein kinases. Liu X, Winey M. Annu Rev Biochem. 2012;81:561-85.

Cytological analysis of Tetrahymena thermophila. Winey M, Stemm-Wolf AJ, Giddings TH Jr, Pearson CG. Methods Cell Biol. 2012;109:357-78.

Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase. Liu C, van Dyk D, Choe V, Yan J, Majumder S, Costanzo M, Bao X, Boone C, Huo K, Winey M, Fisk H, Andrews B, Rao H. J Biol Chem. 2011 Dec 23;286(51):43660-7.

Cell cycle phosphorylation of mitotic exit network (MEN) proteins. Jones MH, Keck JM, Wong CC, Xu T, Yates JR 3rd, Winey M. Cell Cycle. 2011 Oct 15;10(20):3435-40.

The Aurora kinase Ipl1 is necessary for spindle pole body cohesion during budding yeast meiosis. Shirk K, Jin H, Giddings TH Jr, Winey M, Yu HG. J Cell Sci. 2011 Sep 1;124(Pt 17):2891-6.

A cell cycle phosphoproteome of the yeast centrosome. Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR 3rd, Winey M. Science. 2011 Jun 24;332(6037):1557-61.

The two domains of centrin have distinct basal body functions in Tetrahymena. Vonderfecht T, Stemm-Wolf AJ, Hendershott M, Giddings TH Jr, Meehl JB, Winey M. Mol Biol Cell. 2011 Jul 1;22(13):2221-34.

KIF7 mutations cause fetal hydrolethalus and acrocallosal syndromes. Putoux A, Thomas S, Coene KL, Davis EE, Alanay Y, Ogur G, Uz E, Buzas D, Gomes C, Patrier S, Bennett CL, Elkhartoufi N, Frison MH, Rigonnot L, Joyé N, Pruvost S, Utine GE, Boduroglu K, Nitschke P, Fertitta L, Thauvin-Robinet C, Munnich A, Cormier-Daire V, Hennekam R, Colin E, Akarsu NA, Bole-Feysot C, Cagnard N, Schmitt A, Goudin N, Lyonnet S, Encha-Razavi F, Siffroi JP, Winey M, Katsanis N, Gonzales M, Vekemans M, Beales PL, Attié-Bitach T. Nat Genet. 2011 Jun;43(6):601-6.

Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body. Klenchin VA, Frye JJ, Jones MH, Winey M, Rayment I. J Biol Chem. 2011 May 20;286(20):18240-50.

Mip1 associates with both the Mps1 kinase and actin, and is required for cell cortex stability and anaphase spindle positioning. Mattison CP, Stumpff J, Wordeman L, Winey M. Cell Cycle. 2011 Mar 1;10(5):783-93.

Plk4/SAK/ZYG-1 in the regulation of centriole duplication. Pearson CG, Winey M. F1000 Biol Rep. 2010 Aug 9;2:58.

Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodies. Giddings TH Jr, Meehl JB, Pearson CG, Winey M. Methods Cell Biol. 2010;96:117-41.


Basal body stability and ciliogenesis requires the conserved component Poc1. Pearson CG, Osborn DP, Giddings TH Jr, Beales PL, Winey M. J Cell Biol. 2009 Dec 14;187(6):905-20.

High pressure freezing, electron microscopy, and immuno-electron microscopy of Tetrahymena thermophila basal bodies. Meehl JB, Giddings TH Jr, Winey M. Methods Mol Biol. 2009;586:227-41.

Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila. Zweifel E, Smith J, Romero D, Giddings TH Jr, Winey M, Honts J, Dahlseid J, Schneider B, Cole ES. Eukaryot Cell. 2009 Jun;8(6):899-912.

Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation. Holinger EP, Old WM, Giddings TH Jr, Wong C, Yates JR 3rd, Winey M. J Biol Chem. 2009 May 8;284(19):12949-55.

Basal body assembly in ciliates: the power of numbers. Pearson CG, Winey M. Traffic. 2009 May;10(5):461-71.

The two SAS-6 homologs in Tetrahymena thermophila have distinct functions in basal body assembly. Culver BP, Meehl JB, Giddings TH Jr, Winey M. Mol Biol Cell. 2009 Mar;20(6):1865-77.

Basal body components exhibit differential protein dynamics during nascent basal body assembly. Pearson CG, Giddings TH Jr, Winey M. Mol Biol Cell. 2009 Feb;20(3):904-14.

Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules. Gardner MK, Bouck DC, Paliulis LV, Meehl JB, O’Toole ET, Haase J, Soubry A, Joglekar AP, Winey M, Salmon ED, Bloom K, Odde DJ. Cell. 2008 Nov 28;135(5):894-906.


Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle. Crasta K, Lim HH, Giddings TH Jr, Winey M, Surana U. Nat Cell Biol. 2008 Jun;10(6):665-75.

The microtubule-based motor Kar3 and plus end-binding protein Bim1 provide structural support for the anaphase spindle. Gardner MK, Haase J, Mythreye K, Molk JN, Anderson M, Joglekar AP, O’Toole ET, Winey M, Salmon ED, Odde DJ, Bloom K. J Cell Biol. 2008 Jan 14;180(1):91-100.

beta-Catenin is a Nek2 substrate involved in centrosome separation. Bahmanyar S, Kaplan DD, Deluca JG, Giddings TH Jr, O’Toole ET, Winey M, Salmon ED, Casey PJ, Nelson WJ, Barth AI. Genes Dev. 2008 Jan 1;22(1):91-105.

Preventing the degradation of mps1 at centrosomes is sufficient to cause centrosome reduplication in human cells. Kasbek C, Yang CH, Yusof AM, Chapman HM, Winey M, Fisk HA. Mol Biol Cell. 2007 Nov;18(11):4457-69. Epub 2007 Sep 5.

New Tetrahymena basal body protein components identify basal body domain structure. Kilburn CL, Pearson CG, Romijn EP, Meehl JB, Giddings TH Jr, Culver BP, Yates JR 3rd, Winey M. J Cell Biol. 2007 Sep 10;178(6):905-12. Epub 2007 Sep 4. Erratum in: J Cell Biol. 2007 Oct 8;179(1):167.

Centrioles want to move out and make cilia. Pearson CG, Culver BP, Winey M. Dev Cell. 2007 Sep;13(3):319-21.

Mps1 activation loop autophosphorylation enhances kinase activity. Mattison CP, Old WM, Steiner E, Huneycutt BJ, Resing KA, Ahn NG, Winey M. J Biol Chem. 2007 Oct 19;282(42):30553-61.

Physical and functional interaction between mortalin and Mps1 kinase. Kanai M, Ma Z, Izumi H, Kim SH, Mattison CP, Winey M, Fukasawa K. Genes Cells. 2007 Jun;12(6):797-810.

IFT80, which encodes a conserved intraflagellar transport protein, is mutated in Jeune asphyxiating thoracic dystrophy. Beales PL, Bland E, Tobin JL, Bacchelli C, Tuysuz B, Hill J, Rix S, Pearson CG, Kai M, Hartley J, Johnson C, Irving M, Elcioglu N, Winey M, Tada M, Scambler PJ. Nat Genet. 2007 Jun;39(6):727-9.

Centrosome duplication: is asymmetry the clue? Jones MH, Winey M. Curr Biol. 2006 Sep 19;16(18):R808-10.

The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope. Jaspersen SL, Martin AE, Glazko G, Giddings TH Jr, Morgan G, Mushegian A, Winey M. J Cell Biol. 2006 Aug 28;174(5):665-75.

The centrosome cycle. Mattison CP, Winey M. Results Probl Cell Differ. 2006;42:111-46.

Anaphase inactivation of the spindle checkpoint. Palframan WJ, Meehl JB, Jaspersen SL, Winey M, Murray AW. Science. 2006 Aug 4;313(5787):680-4.

Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. Crasta K, Huang P, Morgan G, Winey M, Surana U. EMBO J. 2006 Jun 7;25(11):2551-63.

The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. Araki Y, Lau CK, Maekawa H, Jaspersen SL, Giddings TH Jr, Schiebel E, Winey M. Mol Biol Cell. 2006 Apr;17(4):1959-70.

Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components. Beliakova-Bethell N, Beckham C, Giddings TH Jr, Winey M, Parker R, Sandmeyer S. RNA. 2006 Jan;12(1):94-101.

Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin. Elde NC, Morgan G, Winey M, Sperling L, Turkewitz AP. PLoS Genet. 2005 Nov;1(5):e52. Epub 2005 Nov 4.

Basal body duplication and maintenance require one member of the Tetrahymena thermophila centrin gene family. Stemm-Wolf AJ, Morgan G, Giddings TH Jr, White EA, Marchione R, McDonald HB, Winey M. Mol Biol Cell. 2005 Aug;16(8):3606-19. Epub 2005 Jun 8.

Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena. Bowman GR, Elde NC, Morgan G, Winey M, Turkewitz AP. Traffic. 2005 Apr;6(4):303-23.

Chemical genetics reveals a role for Mps1 kinase in kinetochore attachment during mitosis. Jones MH, Huneycutt BJ, Pearson CG, Zhang C, Morgan G, Shokat K, Bloom K, Winey M. Curr Biol. 2005 Jan 26;15(2):160-5.

Three-dimensional ultrastructure of Saccharomyces cerevisiae meiotic spindles. Winey M, Morgan GP, Straight PD, Giddings TH Jr, Mastronarde DN. Mol Biol Cell. 2005 Mar;16(3):1178-88.

Spindle regulation: Mps1 flies into new areas. Fisk HA, Winey M. Curr Biol. 2004 Dec 29;14(24):R1058-60.

Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. Yoder TJ, McElwain MA, Francis SE, Bagley J, Muller EG, Pak B, O’Toole ET, Winey M, Davis TN. Mol Biol Cell. 2005 Jan;16(1):141-52.

The budding yeast spindle pole body: structure, duplication, and function. Jaspersen SL, Winey M. Annu Rev Cell Dev Biol. 2004;20:1-28.

Cdc28/Cdk1 regulates spindle pole body duplication through phosphorylation of Spc42 and Mps1. Jaspersen SL, Huneycutt BJ, Giddings TH Jr, Resing KA, Ahn NG, Winey M. Dev Cell. 2004 Aug;7(2):263-74.

A novel allele of Saccharomyces cerevisiae NDC1 reveals a potential role for the spindle pole body component Ndc1p in nuclear pore assembly. Lau CK, Giddings TH Jr, Winey M. Eukaryot Cell. 2004 Apr;3(2):447-58.

A field guide to the Mps1 family of protein kinases. Fisk HA, Mattison CP, Winey M. Cell Cycle. 2004 Apr;3(4):439-42.

Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae. Park CJ, Song S, Giddings TH Jr, Ro HS, Sakchaisri K, Park JE, Seong YS, Winey M, Lee KS. Mol Biol Cell. 2004 Apr;15(4):1711-23.

Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression. Fisk HA, Mattison CP, Winey M. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14875-80. Epub 2003 Dec 1.

Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae. Roberts P, Moshitch-Moshkovitz S, Kvam E, O’Toole E, Winey M, Goldfarb DS. Mol Biol Cell. 2003 Jan;14(1):129-41.

Epsilon-tubulin is required for centriole duplication and microtubule organization. Chang P, Giddings TH Jr, Winey M, Stearns T. Nat Cell Biol. 2003 Jan;5(1):71-6.

Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p. Jaspersen SL, Giddings TH Jr, Winey M. J Cell Biol. 2002 Dec 23;159(6):945-56.

Centrosomes and tumour suppressors. Fisk HA, Mattison CP, Winey M. Curr Opin Cell Biol. 2002 Dec;14(6):700-5.

Mutant membrane protein of the budding yeast spindle pole body is targeted to the endoplasmic reticulum degradation pathway. McBratney S, Winey M. Genetics. 2002 Oct;162(2):567-78.

Centrosomes and checkpoints: the MPS1 family of kinases. Winey M, Huneycutt BJ. Oncogene. 2002 Sep 9;21(40):6161-9.

Electron tomography of yeast cells. O’Toole ET, Winey M, McIntosh JR, Mastronarde DN. Methods Enzymol. 2002;351:81-95.

The yeast protein kinase Mps1p is required for assembly of the integral spindle pole body component Spc42p. Castillo AR, Meehl JB, Morgan G, Schutz-Geschwender A, Winey M. J Cell Biol. 2002 Feb 4;156(3):453-65.

Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Burns CG, Ohi R, Mehta S, O’Toole ET, Winey M, Clark TA, Sugnet CW, Ares M Jr, Gould KL. Mol Cell Biol. 2002 Feb;22(3):801-15.

Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Jones MH, He X, Giddings TH, Winey M. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13675-80.

Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit. Luca FC, Mody M, Kurischko C, Roof DM, Giddings TH, Winey M. Mol Cell Biol. 2001 Oct;21(20):6972-83.

Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Giddings TH Jr, O’Toole ET, Morphew M, Mastronarde DN, McIntosh JR, Winey M. Methods Cell Biol. 2001;67:27-42. No abstract available.

Cnm67p is a spacer protein of the Saccharomyces cerevisiae spindle pole body outer plaque. Schaerer F, Morgan G, Winey M, Philippsen P. Mol Biol Cell. 2001 Aug;12(8):2519-33.

The mouse Mps1p-like kinase regulates centrosome duplication. Fisk HA, Winey M. Cell. 2001 Jul 13;106(1):95-104.

Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. Kerscher O, Hieter P, Winey M, Basrai MA. Genetics. 2001 Apr;157(4):1543-53.

Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain. Friedman DB, Kern JW, Huneycutt BJ, Vinh DB, Crawford DK, Steiner E, Scheiltz D, Yates J 3rd, Resing KA, Ahn NG, Winey M, Davis TN. J Biol Chem. 2001 May 25;276(21):17958-67.

The spindle cycle in budding yeast. Winey M, O’Toole ET. Nat Cell Biol. 2001 Jan;3(1):E23-7.

Multi-step control of spindle pole body duplication by cyclin-dependent kinase. Haase SB, Winey M, Reed SI. Nat Cell Biol. 2001 Jan;3(1):38-42.

Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction. Gomez-Ospina N, Morgan G, Giddings TH Jr, Kosova B, Hurt E, Winey M. J Struct Biol. 2000 Oct;132(1):1-5.

Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability. Chial HJ, Stemm-Wolf AJ, McBratney S, Winey M. Curr Biol. 2000 Nov 30;10(23):1519-22.

The spindle checkpoint of Saccharomyces cerevisiae responds to separable microtubule-dependent events. Daum JR, Gomez-Ospina N, Winey M, Burke DJ. Curr Biol. 2000 Nov 2;10(21):1375-8.

Mps1p regulates meiotic spindle pole body duplication in addition to having novel roles during sporulation. Straight PD, Giddings TH Jr, Winey M. Mol Biol Cell. 2000 Oct;11(10):3525-37.


Mechanisms of genetic instability revealed by analysis of yeast spindle pole body duplication. Chial HJ, Winey M. Biol Cell. 1999 Jul;91(6):439-50.

Altered dosage of the Saccharomyces cerevisiae spindle pole body duplication gene, NDC1, leads to aneuploidy and polyploidy. Chial HJ, Giddings TH Jr, Siewert EA, Hoyt MA, Winey M. Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10200-5.

Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Jones MH, Bachant JB, Castillo AR, Giddings TH Jr, Winey M. Mol Biol Cell. 1999 Jul;10(7):2377-91.

Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Muñoz-Centeno MC, McBratney S, Monterrosa A, Byers B, Mann C, Winey M. Mol Biol Cell. 1999 Jul;10(7):2393-406.

Cell cycle: driving the centrosome cycle. Winey M. Curr Biol. 1999 Jun 17;9(12):R449-52. Review.

High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae. O’Toole ET, Winey M, McIntosh JR. Mol Biol Cell. 1999 Jun;10(6):2017-31.

Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. Chial HJ, Rout MP, Giddings TH, Winey M. J Cell Biol. 1998 Dec 28;143(7):1789-800.

Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe. He X, Jones MH, Winey M, Sazer S. J Cell Sci. 1998 Jun;111 ( Pt 12):1635-47.

New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication. Schutz AR, Winey M. Mol Biol Cell. 1998 Apr;9(4):759-74.

DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Komarnitsky SI, Chiang YC, Luca FC, Chen J, Toyn JH, Winey M, Johnston LH, Denis CL. Mol Cell Biol. 1998 Apr;18(4):2100-7.

MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Luca FC, Winey M. Mol Biol Cell. 1998 Jan;9(1):29-46.

Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes. Winey M, Yarar D, Giddings TH Jr, Mastronarde DN. Mol Biol Cell. 1997 Nov;8(11):2119-32.

The yeast CDC37 gene interacts with MPS1 and is required for proper execution of spindle pole body duplication. Schutz AR, Giddings TH Jr, Steiner E, Winey M. J Cell Biol. 1997 Mar 10;136(5):969-82.

Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae. O’Toole ET, Mastronarde DN, Giddings TH Jr, Winey M, Burke DJ, McIntosh JR. Mol Biol Cell. 1997 Jan;8(1):1-11.

Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mathias N, Johnson SL, Winey M, Adams AE, Goetsch L, Pringle JR, Byers B, Goebl MG. Mol Cell Biol. 1996 Dec;16(12):6634-43.

Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Hardwick KG, Weiss E, Luca FC, Winey M, Murray AW. Science. 1996 Aug 16;273(5277):953-6.

Keeping the centrosome cycle on track. Genome stability. Winey M. Curr Biol. 1996 Aug 1;6(8):962-4. Review.

Mapping the three-dimensional organization of microtubules in mitotic spindles of yeast. McDonald K, O’Toole ET, Mastronarde DN, Winey M, Richard McIntosh J. Trends Cell Biol. 1996 Jun;6(6):235-9.

The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. Weiss E, Winey M. J Cell Biol. 1996 Jan;132(1-2):111-23.

Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. Winey M, Mamay CL, O’Toole ET, Mastronarde DN, Giddings TH Jr, McDonald KL, McIntosh JR. J Cell Biol. 1995 Jun;129(6):1601-15.

Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase. Lauzé E, Stoelcker B, Luca FC, Weiss E, Schutz AR, Winey M. EMBO J. 1995 Apr 18;14(8):1655-63.

Yeast beta- and beta’-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. Duden R, Hosobuchi M, Hamamoto S, Winey M, Byers B, Schekman R. J Biol Chem. 1994 Sep 30;269(39):24486-95.

Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Vallen EA, Ho W, Winey M, Rose MD. Genetics. 1994 Jun;137(2):407-22.

Assembly and functions of the spindle pole body in budding yeast. Winey M, Byers B. Trends Genet. 1993 Sep;9(9):300-4.

NDC1: a nuclear periphery component required for yeast spindle pole body duplication. Winey M, Hoyt MA, Chan C, Goetsch L, Botstein D, Byers B. J Cell Biol. 1993 Aug;122(4):743-51.

SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiae. DeMarini DJ, Winey M, Ursic D, Webb F, Culbertson MR. Mol Cell Biol. 1992 May;12(5):2154-64.

MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication. Winey M, Goetsch L, Baum P, Byers B. J Cell Biol. 1991 Aug;114(4):745-54.

The yeast cell cycle. Goebl MG, Winey M. Curr Opin Cell Biol. 1991 Apr;3(2):242-6.